Effect of Ionic Liquid as Mobile-phase Modifier
نویسندگان
چکیده
Studies have been performed to investigate the effect of an ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate, as mobile-phase modifier on the retention behaviour of N-carbobenzyloxy-D-phenylalanine and D-tryptophan on a reversed-phase silica-based column. Retention of these amino acids was determined with water–acetonitrile mobile phases and with mobile phases containing 2, 5, or 10 mmol L ionic liquid. The effect of the amount of ionic liquid modifier on retention was also studied, and interpreted on the basis of intermolecular interactions. The ionic liquid showed promise as an additive in high-performance liquid chromatography.
منابع مشابه
Separation of Guanine and Hypoxanthine with Some Ionic Liquids in RP-HPLC
In this paper, guanine and hypoxanthine were separated with four different ionic liquids as additives for the mobile phase using reversed phase high performance liquid chromatography (RPHPLC). The ionic liquids, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm][BF4]), 1-ethyl-3methylimidazolium tetrafluoroborate ([EMIm][BF4]), 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]) and 1-oct...
متن کاملEffect of ionic liquids as the mobile phase additives on the HPLC resolution of four active compounds from Sophora flavescens Ait.
The retention behaviour of four active compounds from Sophora Flavescens Ait using three ionic liquids as mobile phase modifiers was examined. The effect of the pH and the amount of ionic liquid modifier on the retention of these compounds was determined in methanol/water (v/v) as the mobile phase containing different ionic liquids ranging in concentration from 0.1 mmol/L to 3.0 mmol/L. The ion...
متن کاملSeparation of Sedative – Hypnotic Drugs with Mixed Micellar Liquid Chromatography
Separation of ten sedative- hypnotic drugs was performed by RP-HPLC using mixed micellar mobile phase. Effect of temperature, type and amount of organic modifier in mobile phase on efficiency (N) and asymmetry factor (B/A) showed that, the appropriate conditions for a good separation were 35°C and 7% (V/V) butanol in mobile phase. Variations of selectivity factor versus butanol concent...
متن کاملEVALUATION OF THE USE OF ANIONIC/ NONIONIC MIXED MICELLES IN REVERSED PHASE LIQUID CHROMATOGRAPHY OF CHLOROPHENOLS
The use of aqueous mixed micellar system consisting of sodium dodecyl sulfate (SDS) and polyoxyethylene (23) dodecanol (Brij-35) as the mobile phase in reversed-phase liquid chromatography was studied. A group of chlorophenols was used as the test mixture. Adding organic modifier to the system showed that the use of low concentrations of organic additives improves efficiency in SDS/Brij-35 mixe...
متن کاملEffect of Concentration of Ionic Liquids on Resolution of Nucleotides in Reversed-phase Liquid Chromatography
The chromatographic behaviors of nucleotides (inosine 5'-monophosphate, uridine 5'-monophosphate, guanosine 5'-monophosphate, and thymine monophosphate disodium salts) on a C18 column were studied with different types of ionic liquids (ILs) as additives for the mobile phase in reversed-phase liquid chromatography (RPLC). Three ILs, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm][BF4]), 1-...
متن کامل